Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,095,161 | A→G | L209L (TTA→TTG) | hisC → | histidinol‑phosphate aminotransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,095,161 | 0 | A | G | 99.3% | 389.8 / ‑5.7 | 135 | L209L (TTA→TTG) | hisC | histidinol‑phosphate aminotransferase |
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (134/0); total (135/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
Only 100 of 135 total aligned reads displayed.
GGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCT > W3110S.gb/2095126‑2095187 | ggctggcttgcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:328753/1‑62 (MQ=255) ggctggctggcGGAATATCCGCCCCTGGCTATTTTGCGCACACTGTCGAAAGCtttt > 1:1524056/1‑57 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGt > 1:948893/1‑46 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCg > 1:1408785/1‑48 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGc > 1:1199927/1‑53 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:378632/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:564745/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:550748/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:538909/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:535116/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:509046/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:472079/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:454658/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:452403/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:435498/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:395077/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:627032/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:354819/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:353777/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:341793/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:331550/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:305983/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1021801/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:296755/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:256456/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:813224/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:973184/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:970170/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:936458/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:92924/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:906791/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:903218/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:863255/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:838137/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:81856/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:814774/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:585934/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:804738/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:777827/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:764487/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:724198/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:712661/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:694337/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:688018/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:68512/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:683330/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:30489/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1350800/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1153810/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1393381/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1163435/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1171820/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1359950/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1359579/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:254094/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:135928/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1127683/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1339337/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1323459/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1321784/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1208809/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:124367/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:125067/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1296925/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1266991/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1057455/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1106588/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1467784/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1526354/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1538412/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1548836/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1578865/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:197960/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1603980/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1459142/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1627257/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:193142/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:187730/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1628823/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctct > 1:1639242/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:955155/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:105805/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:857266/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:1098380/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:594561/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:747774/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:727670/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:1303068/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:133632/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:1364217/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:51674/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:504800/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:1579152/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGctc > 1:338376/1‑61 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGct > 1:1255479/1‑60 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTTTGct > 1:1609065/1‑60 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCTTATGctct > 1:1442158/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTGCGCACACTGTCGAAAGCCTTTGctct > 1:240512/1‑62 (MQ=255) ggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGctct > 1:1204288/1‑62 (MQ=255) ggctggctggcGGAATATACGCACCTGGCTATTTTGCGCACACTGTCGAAAGc > 1:5514/1‑53 (MQ=255) | GGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCT > W3110S.gb/2095126‑2095187 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |