Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,118,857	G→T	L136L (CTC→CTA)	wcaL ←	predicted glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,118,857	0	G	T	100.0%	269.4 / NA	95	L136L (CTC→CTA)	wcaL	predicted glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/95);  total (0/95)

CCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTG  >  W3110S.gb/2118810‑2118864                                                |        ccGTGGATGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:240793/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:371384/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:522147/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:505319/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:493458/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:486968/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:473460/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:466969/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:466185/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:448315/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:437912/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:427992/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:386826/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:54896/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:361543/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:361497/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:35467/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:326095/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:309451/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:305553/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:303338/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:301559/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:30081/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:102199/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:821758/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:99720/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:987815/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:935459/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:890529/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:886485/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:880927/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:876737/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:868584/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:847924/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:843424/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:839998/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:285410/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:812188/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:811983/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:779354/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:754195/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:741307/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:681950/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:678500/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:642100/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:600812/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:553028/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1249769/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:142623/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:14158/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1412076/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1391291/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1390597/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1383263/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:135628/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1311405/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1297983/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1266247/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:267169/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1173542/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1140463/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1136978/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1122025/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1090820/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1078700/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1055633/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1045591/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1037285/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1029337/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1547019/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:274003/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:261666/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:261382/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:199291/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:167060/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:166697/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1607401/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1593894/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1561765/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1560025/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1559254/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1432242/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1543531/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1526854/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1508849/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1499093/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1487418/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:147826/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1467406/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:146564/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1449362/55‑1 (MQ=255) ccGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGATCGCGTAGTTTtg  <  1:981753/55‑1 (MQ=255) ccGTGGAAGAAAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:742966/55‑1 (MQ=255)  cGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:773250/54‑1 (MQ=255)               ggCAATTTTGCCGCGAATGACACCCAGTTCGCGTAGTTTtg  <  1:1422083/41‑1 (MQ=255)                                                |        CCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTG  >  W3110S.gb/2118810‑2118864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑