Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,192,625 C→A Q540H (CAG→CAT yehB ← predicted outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,192,6250CA86.5% 702.2 / 93.1 310Q540H (CAG→CATyehBpredicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (18/24);  new base A (268/0);  total (286/24)
Fisher's exact test for biased strand distribution p-value = 8.82e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

GATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCCGCGAGGGTATAGCTTATCCGTCGCAGGTTGTTGGAATAACTCAA  >  W3110S.gb/2192575‑2192678
                                                  |                                                     
gATATAAAAATATTAAAACGTTTCTCTTCATGATGCTTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:1614570/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:541887/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:1216842/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:151033/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:1306178/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:344449/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:1048782/62‑1 (MQ=255)
gATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCcgc                                            <  1:973899/62‑1 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGc                                 >  1:355193/1‑35 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGc                                 >  1:1468463/1‑35 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGc                                 >  1:1531887/1‑35 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:491123/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:517850/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:477802/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:527987/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:472160/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:458195/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:420448/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:440771/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:430640/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:574474/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:411204/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:397743/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:390255/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:372020/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:320559/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:313942/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:301410/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:292406/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:753091/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:977361/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:957048/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:940613/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:938008/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:930560/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:919419/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:901276/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:86215/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:842257/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:797787/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:761935/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:550223/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:738545/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:724637/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:694750/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:688980/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:672035/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:653153/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:628652/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:608776/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:57840/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:233301/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:562115/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1181264/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1355333/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1325711/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1317211/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1294346/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1285825/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1261172/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1204444/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:120058/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1197649/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1195556/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:261352/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1171566/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:115356/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1134021/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:11153/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:110323/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1092117/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1084298/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1061853/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1041258/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1012928/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1567507/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:276163/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:247630/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1003873/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:19806/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:195160/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:182184/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:166343/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1634144/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1604076/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1587766/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1383653/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1558553/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:152557/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1518842/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1474891/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1461263/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1454194/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1443213/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:1402598/1‑36 (MQ=255)
                                      ctcGTCATAAGCATGGCTTGCCGCGAGGGGATAGCt                                >  1:1497919/1‑36 (MQ=38)
                                      ctcGTCAAAAGCATGGCTTGCCGCGAGGGTATAGCt                                >  1:130972/1‑36 (MQ=38)
                                          tCATAAGCCTGGCTTGCCGCGAGGGTATAGCTTATCCGTCGCAGGTTGTTGGAATAACTCaa  >  1:1176088/1‑62 (MQ=255)
                                          tCATAAGCCTGGCTTGCCGCGAGGGTATAGCTTATCCGTCGCAGGTTGTTGGAATAACTCaa  >  1:856023/1‑62 (MQ=255)
                                          tCATAAGCCTGGCTTGCCGCGAGGGTATAGCTTATCCGTCGCAGGTTGTTGGAATAACTCa   >  1:133914/1‑61 (MQ=255)
                                                  |                                                     
GATATAAAAATATTAAAACGTTTCTCTTCATGATGATTCTCGTCATAAGCCTGGCTTGCCGCGAGGGTATAGCTTATCCGTCGCAGGTTGTTGGAATAACTCAA  >  W3110S.gb/2192575‑2192678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: