Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,229,236	G→A	A34A (GCG→GCA)	yohD →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,229,236	0	G	A	100.0%	125.6 / NA	46	A34A (GCG→GCA)	yohD	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/0);  total (46/0)

GAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGT  >  W3110S.gb/2229204‑2229265                                 |                              gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCa                          >  1:1361614/1‑38 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTGAAGTTCCCGCtggt  >  1:384721/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:596216/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:186273/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:227149/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:248437/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:30329/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:310710/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:3350/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:401199/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:410593/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:455436/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:535945/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:177557/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:626171/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:672631/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:745945/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:824136/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:830424/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:944637/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:949990/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:97686/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:995461/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:146657/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1010781/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1028055/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1075677/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1075995/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:111471/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1261314/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1264814/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1272530/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1323235/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1383007/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1005885/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1469332/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1556038/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1593489/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1607274/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1623563/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1624132/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1627984/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1646690/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtgg   >  1:159995/1‑61 (MQ=255) gAAACCGTGACTTTGCTGGGAGGCGTTGCGGAACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:430176/1‑62 (MQ=255) gAAACCGTGACTTTGCTGGGAGGAGTTGCGGCACATCAGGGGCTATTAAAGTTCCCGCtggt  >  1:1461874/1‑62 (MQ=255)                                 |                              GAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGT  >  W3110S.gb/2229204‑2229265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑