Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,274,320 A→G A87A (GCA→GCG)  rtn → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,274,3200AG99.5% 616.7 / NA 211A87A (GCA→GCG) rtnconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (210/0);  minor base T (1/0);  total (211/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

AGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGC  >  W3110S.gb/2274289‑2274329
                               |         
aGCAGGCTAACCCTGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1112410/1‑41 (MQ=37)
aGCAGGCTAACCCCGAACTGACCGCCCGCgcggcg        >  1:1624174/1‑35 (MQ=37)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTa    >  1:1390332/1‑39 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTa    >  1:702295/1‑39 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTa    >  1:917051/1‑39 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAg   >  1:294137/1‑40 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:488542/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:559594/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:609858/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:548042/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:536710/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:525126/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:516969/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:509042/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:504567/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:320443/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:495889/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:366148/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:480621/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:472415/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:348845/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:455537/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:429244/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:419073/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:417490/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:359021/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:409081/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:37429/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:783396/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:991234/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:969727/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:886210/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:870912/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:864954/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:853858/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:850872/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:840807/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:836783/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:820161/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:802524/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:595426/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:774513/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:769210/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:763877/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:735566/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:698642/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:690109/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:68566/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:648763/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:644892/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:276944/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1189653/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1371166/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1354766/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1347868/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1335497/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1333085/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1325732/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1307436/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1295038/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:127375/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1236040/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1209567/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1410498/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:118292/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:116450/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1137451/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1081906/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1069353/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1062130/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1056688/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1051270/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1036700/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1035530/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1030870/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1425533/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:274599/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:24813/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:23822/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:231078/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:226052/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:220600/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:196052/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:189532/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1642895/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1634902/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1607862/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1578821/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1552227/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1540397/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1527105/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1497262/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1482398/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1474742/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1457232/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:28568/1‑41 (MQ=38)
aGCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGATTAGc  >  1:29866/1‑41 (MQ=37)
aGCAGGCTAACCCCGAACTGACAGCCCGCGCGGCGTTTAGc  >  1:1161138/1‑41 (MQ=37)
aGCACGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1510965/1‑41 (MQ=38)
 gCAGGCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGc  >  1:1002596/1‑40 (MQ=38)
                               |         
AGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGC  >  W3110S.gb/2274289‑2274329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑