Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,330,754 C→A intergenic (+6/‑25) atoE → / → atoB short chain fatty acid transporter/acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,330,7540CA100.0% 585.9 / NA 209intergenic (+6/‑25)atoE/atoBshort chain fatty acid transporter/acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (209/0);  total (209/0)

Only 100 of 212 total aligned reads displayed.

GTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACA  >  W3110S.gb/2330716‑2330761
                                      |       
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAaccc      >  1:1253019/1‑42 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTAc   >  1:765788/1‑45 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:458756/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:612639/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:59601/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:585795/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:560586/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:558120/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:546793/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:540007/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:530253/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:498177/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:494721/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:484859/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:625900/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:43785/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:399289/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:375594/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:350643/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:315226/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:285539/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:275988/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:263560/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:229840/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:221821/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:188559/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:184284/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:783873/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:990310/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:961783/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:933811/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:917517/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:902843/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:895195/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:885811/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:868178/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:854616/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:849383/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:823760/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:624011/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:781290/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:762872/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:755367/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:737864/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:736000/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:728261/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:68781/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:673130/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:655058/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:634409/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:17775/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1168975/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1346249/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1331931/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1321038/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1311376/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1302623/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1295562/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1279739/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1271736/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1227818/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1205268/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1188423/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1352898/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1162096/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1159483/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1138436/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1113866/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:110433/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1075150/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1067045/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1055285/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1047393/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1042965/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1018713/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1539944/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:167097/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1646982/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1635454/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1621146/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1617407/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1609157/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1597365/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1595224/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1586715/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1559139/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1550079/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1544130/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1005504/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1523004/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1499747/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1487389/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1447718/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1432322/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1416660/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1381614/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1378742/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1373793/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCTACa  >  1:1360538/1‑46 (MQ=255)
gtCATTTTCGTCATTGGTTTAACGCTGTTCAGACGGCAACCCTACa  >  1:1229488/1‑46 (MQ=255)
                                      |       
GTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACA  >  W3110S.gb/2330716‑2330761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑