Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,437,879	C→A	A170A (GCG→GCT)	folC ←	bifunctional folylpolyglutamate synthase and dihydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,437,879	0	C	A	100.0%	86.2 / NA	32	A170A (GCG→GCT)	folC	bifunctional folylpolyglutamate synthase and dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/0);  total (32/0)

TGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAA  >  W3110S.gb/2437822‑2437883                                                          |     tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1639164/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:962136/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:908654/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:829009/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:822945/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:821018/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:698960/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:409332/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:354806/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:345840/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:343755/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:309639/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:30132/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:233754/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:176553/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:167833/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1082818/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:162445/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1492974/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:14692/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1462000/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1457159/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1417502/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1392020/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1366986/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1337706/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1332328/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1302500/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1175193/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1119897/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGAGCac  >  1:1113858/1‑61 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCAAGAGCac  >  1:1543994/1‑61 (MQ=255)                                                          |     TGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAA  >  W3110S.gb/2437822‑2437883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑