Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,441,808	G→T	S96R (AGC→AGA)	usg ←	predicted semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,441,808	0	G	T	100.0%	93.3 / NA	34	S96R (AGC→AGA)	usg	predicted semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/0);  total (34/0)

CGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAA  >  W3110S.gb/2441789‑2441848                    |                                         cGAGAGTAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1283494/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGTCAACCTGAGTTGGTCGCTTCTTCaa  >  1:3599/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTg               >  1:1422052/1‑47 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:588188/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:209252/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:225290/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:281430/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:340091/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:36472/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:366679/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:56589/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1638619/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:732926/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:791559/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:844737/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:892976/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:984688/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:987819/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1056143/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1631125/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1606357/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1582825/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1538858/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1485159/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1457247/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1453033/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1436354/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1431833/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1366459/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1304216/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1208437/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:119879/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa  >  1:1188642/1‑60 (MQ=255) cGAGAGCAAACAATCCACTTCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCa   >  1:947665/1‑59 (MQ=255)                    |                                         CGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAA  >  W3110S.gb/2441789‑2441848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑