Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,659,216 T→G S324S (TCA→TCC)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,659,2160TG100.0% 439.5 / NA 150S324S (TCA→TCC) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (150/0);  total (150/0)

Only 100 of 150 total aligned reads displayed.

GTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCA  >  W3110S.gb/2659177‑2659238
                                       |                      
gTAGGACGGTTCGAGGCTTGCTGCCGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:213706/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACcgcg            >  1:1027373/1‑52 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCTAGGTCt      >  1:266689/1‑58 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1007193/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:546482/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:705/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:756507/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1155257/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:15954/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:24864/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1108496/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:379534/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:22422/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:525132/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:492848/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:483007/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:464368/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:462128/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:445148/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:426157/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:992394/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:404645/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:363724/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:356158/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:344243/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:338259/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:254502/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:330125/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:292832/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:327271/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:782488/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1005391/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:981515/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:965539/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:960826/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:957532/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:932985/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:914111/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:859238/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:834171/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:801402/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:787266/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:56843/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:772263/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:729727/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:725278/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:681668/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:661852/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:634690/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:621728/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:607620/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:593242/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:569109/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1162120/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1313832/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1308471/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1294875/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1267002/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1265716/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1219314/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1214028/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1205057/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1203600/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1197419/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1195340/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1328856/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1160641/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1149402/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1141731/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1122750/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1106709/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1098020/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1071801/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1042122/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1032003/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1020041/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1505998/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:213572/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:194318/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1639928/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1629660/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1592464/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1561403/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1546419/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1533055/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1513081/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1508279/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:216661/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1478028/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:146614/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1454868/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1440637/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1401420/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1398385/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1396317/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1392955/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1388600/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1377778/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCCGAACCGGAAGAAACCGCGAGCTCTTTCa  >  1:674813/1‑62 (MQ=255)
gTAGGACGGTTCGAGGATTGCTGACGTACAGGCGGAACCGGAAGAAAcct              >  1:18090/1‑48 (MQ=255)
                                       |                      
GTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCA  >  W3110S.gb/2659177‑2659238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑