Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,659,225 T→C A321A (GCA→GCG)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,659,2250TC100.0% 421.0 / NA 147A321A (GCA→GCG) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (147/0);  total (147/0)

Only 100 of 148 total aligned reads displayed.

GTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCA  >  W3110S.gb/2659177‑2659238
                                                |             
gTAGGACGGTTCGAGGCTTGCTGCCGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:213706/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACATGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1392148/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACcgcg            >  1:1027373/1‑52 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCt      >  1:11467/1‑58 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1007193/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:258976/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:313194/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1490302/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1309762/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:705/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:756507/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1199241/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1155257/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1031762/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1057950/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTc   >  1:1108496/1‑61 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:379534/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:525132/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:50272/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:981515/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:483007/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:469577/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:462128/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:450700/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:426157/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:416234/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:56843/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:375230/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:356158/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:344243/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:334883/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:330125/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1005391/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:292832/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:992394/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:725278/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:914111/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:859238/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:834171/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:801402/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:957532/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:787266/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:782488/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:772263/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:729727/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:549136/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:960826/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:965539/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:661852/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:634690/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:621728/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:607620/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:593242/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:580603/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:932985/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1205057/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:254502/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1377778/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1348993/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1313832/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1308471/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1287473/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1267002/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1265260/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1219314/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1209603/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1392955/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1197419/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:118043/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1162120/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1157219/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1141731/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1118659/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1106224/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1098020/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1042122/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1013606/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1540056/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:237280/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:22422/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:214075/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:213572/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:194318/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1640304/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1629660/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1595639/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1592464/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1579986/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1546419/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:139647/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1513081/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1510516/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1505998/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:146614/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1458076/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1440637/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1428703/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTTTCa  >  1:1398385/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCGGAAGAAACCGCGAGGTCTATCa  >  1:684008/1‑62 (MQ=255)
gTAGGACGGTTCGAGGCTTGCTGACGTACAGGCCGAACCGGAAGAAACCGCGAGCTCTTTCa  >  1:674813/1‑62 (MQ=255)
                                                |             
GTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCA  >  W3110S.gb/2659177‑2659238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑