Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 215,640 C→T G124G (GGC→GGT)  nlpE → lipoprotein involved with copper homeostasis and adhesion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb215,6400CT100.0% 608.9 / NA 210G124G (GGC→GGT) nlpElipoprotein involved with copper homeostasis and adhesion
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (210/0);  total (210/0)

Only 100 of 210 total aligned reads displayed.

CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTT  >  W3110S.gb/215632‑215693
        |                                                     
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGATCCGGCACAATCCAGctt  >  1:121887/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACATTCCAGctt  >  1:589012/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct   >  1:1205644/1‑59 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgc    >  1:1046648/1‑59 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:399360/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:535103/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:51088/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:506806/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:500843/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:489253/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:464351/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:451391/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:429482/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:417869/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:412566/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:23085/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:392412/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:375544/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:361443/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:338935/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:287955/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:279435/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:274504/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:261715/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:258628/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:250764/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:243502/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:216470/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:802150/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:978917/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:976447/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:958821/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:956026/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:948206/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:934289/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:907213/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:90624/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:896112/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:8681/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:837399/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:549230/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:779837/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:766072/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:744056/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:736727/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:705661/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:672049/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:656698/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:641113/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:630367/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:593049/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:56892/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1167697/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1334172/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1327967/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1325674/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1322003/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1311380/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:129548/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1283242/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1270577/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1238265/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1213166/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1201119/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1360879/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1160718/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1155384/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1147824/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1126955/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1117204/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1101572/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1085618/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1062891/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1034823/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1031595/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1025241/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1537295/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1010231/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:198673/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:173483/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1643987/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1628748/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1624035/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1613695/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1610859/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:159083/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1573211/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1557338/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:224468/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1522931/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:150004/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:14923/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1478909/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1454346/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1433816/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1407348/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1404590/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1391268/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1374115/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt  >  1:1368546/1‑62 (MQ=255)
        |                                                     
CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTT  >  W3110S.gb/215632‑215693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑