Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,679,929 G→A D491D (GAC→GAT)  yphG ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,679,9290GA100.0% 720.8 / NA 250D491D (GAC→GAT) yphGconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (250/0);  total (250/0)

Only 100 of 250 total aligned reads displayed.

ATAATCCAGCGGGTCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGC  >  W3110S.gb/2679917‑2679972
            |                                           
aTAATCCAGCGGATCCGGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1388786/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGgtagt                    >  1:650814/1‑38 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGgt                       >  1:158017/1‑35 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACgg   >  1:1646753/1‑55 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:57633/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:590650/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:555444/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:53865/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:533730/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:520358/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:518137/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:504368/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:500205/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:466073/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:437154/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:420669/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:409117/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:371930/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:368750/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:340213/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:331444/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:320194/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:311119/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:296653/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:290034/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:270410/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:256816/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:748286/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:971808/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:966220/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:915868/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:909149/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:886081/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:870080/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:857168/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:848785/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:828537/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:81335/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:777778/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:754307/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:230497/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:743375/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:717144/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:699666/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:6804/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:679441/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:658836/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:636484/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:630769/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:618801/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:601149/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:592467/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1191605/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:232976/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1325690/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1302948/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1294369/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1289927/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1283931/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1280667/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1271187/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1233628/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1215172/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1203008/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1332177/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1169730/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1160079/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:113639/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1128051/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1096481/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:107769/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1062454/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1035510/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1035335/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:102476/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1553403/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:101819/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:213606/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:197119/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:19154/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:189464/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:179742/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:176719/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1638080/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1613526/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1610145/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1577865/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1347903/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1533980/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1499599/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1481305/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1454068/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:144949/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1434799/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1424547/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1397044/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1372358/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGc  >  1:1361527/1‑56 (MQ=255)
aTAATCCAGCGGATCCAGCGCCACGCCGCGCAGGTAGTAATAGAACGGTGAACgg   >  1:1071878/1‑55 (MQ=39)
aTAATCCAGCGGATCCAGCGCCACGCCACGCAGGTAGTAATCGa              >  1:1317551/1‑44 (MQ=38)
            |                                           
ATAATCCAGCGGGTCCAGCGCCACGCCGCGCAGGTAGTAATCGAACGGTGAACGGC  >  W3110S.gb/2679917‑2679972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑