Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,691,044	C→G	S1052S (TCG→TCC)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,691,044	0	C	G	100.0%	256.0 / NA	89	S1052S (TCG→TCC)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/89);  total (0/89)

TCAACATGCGAGTTCACGCCCTGCTCACGCAGTACGGCAACTTTCGGACGTGCGCCAGTGGC  >  W3110S.gb/2691036‑2691097         |                                                      tCAACATGGGAGTTCACAGCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1421552/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTTGGGCGCGCGCCAGTGGc  <  1:823321/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCATTGGc  <  1:1471765/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:39743/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:626202/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:606017/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:588339/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:582715/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:54948/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:471054/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:449363/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:415515/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:408486/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:630331/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:385997/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:348935/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:346573/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:342640/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:31740/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:305425/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:279642/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:262661/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:260632/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:807271/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:982770/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:951693/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:947697/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:935578/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:931236/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:915571/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:875103/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:871512/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:817902/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:809476/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:241591/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:790834/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:739470/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:730635/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:722097/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:720998/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:716412/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:714726/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:708821/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:656880/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1269877/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1093067/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1379737/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1356697/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1334458/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1328798/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1292245/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1288604/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1287529/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1279980/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1277314/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1397289/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1165797/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1154231/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1148759/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1143925/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1135011/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:112219/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1119987/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1115863/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1098179/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1093784/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1560293/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:215471/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:166808/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1638215/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1634033/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:160422/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:226828/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1592611/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1564416/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1415639/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:153325/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1533027/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1532713/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1529932/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:235867/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1482056/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1477818/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1428179/62‑1 (MQ=255) tCAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:237940/62‑1 (MQ=255) tCAACATGGCAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:398175/62‑1 (MQ=255)  cAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:1151029/61‑1 (MQ=255)  cAACATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:565955/61‑1 (MQ=255)   aaCATGGGAGTTCACACCCTGCTCACGCAGTACGGCAACTTTCGGGCGCGCGCCAGTGGc  <  1:641432/60‑1 (MQ=255)         |                                                      TCAACATGCGAGTTCACGCCCTGCTCACGCAGTACGGCAACTTTCGGACGTGCGCCAGTGGC  >  W3110S.gb/2691036‑2691097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑