Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,737,465	G→T	M355I (ATG→ATT)	pheA →	fused chorismate mutase P and prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,737,465	0	G	T	100.0%	72.3 / NA	27	M355I (ATG→ATT)	pheA	fused chorismate mutase P and prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/0);  total (27/0)

GATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAAT  >  W3110S.gb/2737433‑2737494                                 |                              gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:307217/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:876332/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:829082/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:733876/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:731977/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:728036/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:695767/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:679219/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:604659/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:517780/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:509582/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:492160/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:482016/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:413374/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1008696/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:278271/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:225341/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1637048/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1580262/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1504658/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1465608/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1433648/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:128294/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1271054/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1188902/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1162151/1‑62 (MQ=255) gATATTCAGGCCAATCTTGAATCAGCGGAAATTCAAAAAGCATTGAAAGAGTTAGGGGAAAt  >  1:1158388/1‑62 (MQ=255)                                 |                              GATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAAT  >  W3110S.gb/2737433‑2737494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑