Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 215,667 T→C Y133Y (TAT→TAC)  nlpE → lipoprotein involved with copper homeostasis and adhesion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb215,6670TC75.8% 418.4 / 123.0 277Y133Y (TAT→TAC) nlpElipoprotein involved with copper homeostasis and adhesion
Reads supporting (aligned to +/- strand):  ref base T (0/67);  new base C (210/0);  total (210/67)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 279 total aligned reads displayed.

CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGC  >  W3110S.gb/215632‑215704
                                   |                                     
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGATCCGGCACAATCCAGctt             >  1:121887/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGcaca                      >  1:954045/1‑53 (MQ=38)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCTCAATCCAGctt             >  1:498441/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct              >  1:58297/1‑59 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct              >  1:906772/1‑59 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgc               >  1:1046648/1‑59 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:250764/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:501715/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:451391/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:429482/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:417869/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:405046/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:375544/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:299091/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:271691/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:25868/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1622455/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:241683/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:224468/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1010231/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:198673/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:173483/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1643987/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1628748/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:216470/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:766072/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:985838/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:977318/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:973153/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:956026/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:934289/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:896112/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:837399/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:80744/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:781923/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:51088/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:745888/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:738526/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:658574/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:644323/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:640493/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:626702/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:593049/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:556852/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:535103/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1160718/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1368546/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1360879/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1334172/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1610859/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1324496/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1311380/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:129548/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1283242/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1237804/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1201623/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1327967/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1154957/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1147824/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1134005/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:111918/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1101572/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1088967/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1062891/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1034823/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1022014/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:150075/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1408910/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1374115/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1433816/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1454346/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1478909/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1499578/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1537295/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1550149/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1573211/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1397604/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:1584965/1‑62 (MQ=255)
cGTGAAGGTAATCCGATTGAATCGCAGTTAAACTACACGCTGTAACCGGCACAAACCAgcta             >  1:842093/1‑59 (MQ=255)
cGTGAAGGTAATACGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt             >  1:484591/1‑62 (MQ=255)
          aTCCGATTGAATCGCAGTTCAACTATACGCTGGAAG‑GGCACAATCCAGTTTACCTATGACGc  <  1:462202/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:676712/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:236566/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1197610/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1027306/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:708836/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:277382/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1262484/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1271345/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:28137/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:140656/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:317095/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:360838/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:395120/62‑1 (MQ=255)
           tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1378132/62‑1 (MQ=255)
            ccGATTGAATCTCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1177785/61‑1 (MQ=255)
            ccGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1207386/61‑1 (MQ=255)
            ccGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:568997/61‑1 (MQ=255)
                             cAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:342930/44‑1 (MQ=255)
                                 tataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc  <  1:1252969/40‑1 (MQ=255)
                                   |                                     
CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGC  >  W3110S.gb/215632‑215704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑