Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,869,895 G→C A22A (GCC→GCG)  truD ← pseudoruidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,869,8950GC90.2% 487.7 / 29.9 184A22A (GCC→GCG) truDpseudoruidine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/18);  new base C (0/166);  total (0/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCG  >  W3110S.gb/2869860‑2869928
                                   |                                 
cccAAATCTTCCACCTCCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:598729/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCTGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1298437/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:351554/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:469243/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:464764/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:449655/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:428301/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:389831/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:38084/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:372342/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:369640/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:355309/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:519824/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:305742/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:27633/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:266776/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:263062/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:248244/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:221389/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:216858/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:201798/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:189469/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:188208/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:177251/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:723756/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:975968/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:939889/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:932229/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:921193/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:88194/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:866469/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:828276/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:807864/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:795384/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:763654/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:481068/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:690650/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:65946/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:629454/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:570454/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:56554/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:55537/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:546919/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:530082/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:20704/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1206485/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1368705/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1629785/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1317913/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1310754/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1300628/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1293958/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1261683/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1254003/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:12074/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1351950/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1199523/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1189105/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1179804/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1145192/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1131426/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1117175/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1113576/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1089281/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1024725/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1494450/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1603205/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1595052/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1581154/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1573774/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1540875/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1523383/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1392725/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1442676/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1431994/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1394303/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:14142/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCAGTGCCTTGCgg         <  1:456270/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTACAGCAGCCCGGTGCCTTGCgg         <  1:951772/62‑1 (MQ=255)
cccAAATCTTCCACCACCACAAACTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1600813/62‑1 (MQ=39)
cccAAATCTTCCACAACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:238439/62‑1 (MQ=255)
 ccAAATCTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:971873/61‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:649635/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1401885/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:77473/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1069529/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:301191/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:889177/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1274283/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:924214/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1407470/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCAGTGCCTTGCGGTTTACCg  <  1:1472775/62‑1 (MQ=255)
       cTTCCACCACCACAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1363305/55‑1 (MQ=255)
           caccaccacAAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGACTTGCgg         <  1:1001957/51‑1 (MQ=39)
                  acaAAGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:471128/44‑1 (MQ=255)
                    aaaGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1381176/42‑1 (MQ=255)
                     aaGTCTTCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1171390/41‑1 (MQ=255)
                       gTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1225106/46‑1 (MQ=255)
                          ttCCGGATTCGCTTTCAGCAGCCCGGTGCCTTGCgg         <  1:1551709/36‑1 (MQ=255)
                            ccGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCg  <  1:1358632/41‑1 (MQ=255)
                                   |                                 
CCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCG  >  W3110S.gb/2869860‑2869928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑