Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,917,055 G→A C329C (TGC→TGT)  gudD ← (D)‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,917,0550GA98.2% 295.3 / ‑6.2 109C329C (TGC→TGT) gudD(D)‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (107/0);  minor base C (2/0);  total (109/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGAT  >  W3110S.gb/2917043‑2917104
            |                                                 
gCCAAATTCATGCCACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCg     >  1:19100/1‑59 (MQ=39)
gCCAAATTCATGCCACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:179467/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCCCACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:823866/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACCCGTACCGAACCTTGCATCGTc               >  1:1158596/1‑49 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACCCGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1200651/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGCACCTTGCATCGTCCAGAAATGCgg    >  1:1172716/1‑60 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCAtcg                 >  1:95468/1‑45 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGaa          >  1:1401294/1‑54 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGa           >  1:142702/1‑53 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCgg    >  1:490531/1‑60 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCgg    >  1:487798/1‑60 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:1461265/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:738479/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:1457046/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:801935/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:646739/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:1452359/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:535635/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:882994/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:1023316/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:471577/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGa   >  1:1381082/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:446839/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:446583/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:335082/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:359817/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:352583/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:546162/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:29477/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:244471/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:216961/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1628029/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1623381/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1598336/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1572358/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:803538/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:970640/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:967854/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:964255/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:961723/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:9529/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:952128/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:938809/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:853929/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:80758/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:485169/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:753256/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:731248/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:67741/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:609818/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:579476/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:557912/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1547788/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:490877/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:12089/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1571949/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1297937/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1297315/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1283753/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1279904/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1276338/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1269074/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1266429/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1263346/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1209912/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1298499/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1202155/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1132370/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1111643/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1104387/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1101251/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1076642/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1063240/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1047127/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:101674/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1003276/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1453479/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1556312/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1548651/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1001537/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1526337/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1517383/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1508561/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1485449/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1476575/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1465280/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1299925/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1443617/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1434601/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1408160/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1349867/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1339668/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1329316/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1318866/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1313643/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1313478/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:1309115/1‑62 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACCTTGCATCGTACAGAAATGCGGa   >  1:729617/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCCACACGTACCGAACATTGCATCGTCCAGAAATGCGGa   >  1:182706/1‑61 (MQ=255)
gCCAAATTCATGACACATTTGCGCAACACGTACCGAACCTTGCATCGTCCAGAAATGCGGAt  >  1:961492/1‑62 (MQ=255)
            |                                                 
GCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGAT  >  W3110S.gb/2917043‑2917104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑