Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,945,260 C→A G192V (GGC→GTC)  mltA ← membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,945,2600CA100.0% 360.6 / NA 128G192V (GGC→GTC) mltAmembrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (126/2);  total (126/2)

Only 100 of 128 total aligned reads displayed.

GTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACC  >  W3110S.gb/2945223‑2945288
                                     |                            
gTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCAc      <  1:1059017/62‑1 (MQ=255)
gTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCAc      <  1:1068182/62‑1 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTTACATCACCAAAGTCGATATACCCACTAc   >  1:820995/1‑61 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTc                 >  1:1033288/1‑47 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCGCTAcc  >  1:733298/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:415382/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:345598/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:347226/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:353342/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:37268/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:383174/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:383482/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:514845/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:434990/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:438860/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:473391/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:527899/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:295525/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:258713/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:223407/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:223140/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:213156/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:20692/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:20328/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:199232/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1634475/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1633276/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1016017/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:691327/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:967648/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:933958/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:896364/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:877681/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:86160/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:772824/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:769809/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:769753/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:741167/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:724714/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:520002/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:690765/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:644982/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:606641/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:591790/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:58792/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:575825/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:564962/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:532683/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1630528/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:521325/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1164621/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1323417/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1625767/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1309622/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1306026/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1296436/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1276935/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1274549/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1241910/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1238796/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1197819/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1194660/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1321500/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1128610/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1116169/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1113579/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1107950/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1102196/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1090911/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1070982/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1060567/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:102983/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1016020/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1335333/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1613321/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1611873/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1604224/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1587733/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:157370/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:156562/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1548458/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1543490/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1541264/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1491109/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:148681/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1469785/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1461439/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1430622/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1421230/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:139801/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1392319/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1388459/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1373855/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAcc  >  1:1334912/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAc   >  1:1522296/1‑61 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAc   >  1:1090412/1‑61 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACCCACTAc   >  1:946758/1‑61 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACACACTAcc  >  1:704285/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACACACTAcc  >  1:72215/1‑62 (MQ=255)
    ttCCCTGCATAGCTGAAAAAGTTAAGCGGACTGACATCACCAAAGTCGATATACACACTAcc  >  1:203748/1‑62 (MQ=255)
                                     |                            
GTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACC  >  W3110S.gb/2945223‑2945288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑