Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,977,793 T→G L39L (CTT→CTG)  lysR → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,977,7930TG100.0% 565.3 / NA 192L39L (CTT→CTG) lysRDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (192/0);  total (192/0)

Only 100 of 192 total aligned reads displayed.

ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAA  >  W3110S.gb/2977762‑2977823
                               |                              
accccTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1061611/4‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTgag                    >  1:444811/1‑44 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGaa                  >  1:1254293/1‑46 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGaa                  >  1:462376/1‑46 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:347634/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:540775/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:519853/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:502719/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:471785/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:469809/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:446053/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:422898/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:362055/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1614982/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:338365/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:328888/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:277972/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:258386/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:239834/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:228094/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1001887/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:177270/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1643511/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:218636/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:785819/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:995443/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:939131/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:926745/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:918185/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:89248/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:875370/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:830992/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:82132/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:799207/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:786354/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:562203/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:775680/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:75124/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:740622/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:692365/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:68150/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:64665/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:596415/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:589926/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:563816/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:119175/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1341666/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1304334/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1285380/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1277534/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1566058/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1243236/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1233282/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1220011/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1210574/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1209592/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1275686/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:11608/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1138961/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1127559/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1073268/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1069024/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1046460/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1023356/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1020460/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1010056/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1005614/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1376328/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:156163/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1544694/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1538956/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1524360/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1518414/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1496580/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:144738/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1368648/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1428006/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1391305/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1424720/1‑62 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1121042/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:964664/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:958087/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1285105/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:196030/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1032350/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1530716/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:416352/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1079238/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1508546/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:26503/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:1501018/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:726963/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:725645/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:70924/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:632177/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:574342/1‑61 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGa    >  1:318738/1‑60 (MQ=255)
acacCTCACAGCCAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTCATCGGGCTGaaa  >  1:1484631/1‑62 (MQ=255)
acacCTCACAGCCAAACGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaa   >  1:353377/1‑61 (MQ=255)
acacCTCACAGACAACCGTCAGCCGCGAACTGGCGCGCTTTGAGAAGGTGATCGGGCTGaaa  >  1:1041751/1‑62 (MQ=255)
                               |                              
ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAA  >  W3110S.gb/2977762‑2977823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑