Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,013,669	A→G	G15G (GGT→GGC)	yqeC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,013,669	0	A	G	100.0%	100.5 / NA	36	G15G (GGT→GGC)	yqeC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/0);  total (36/0)

GCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACC  >  W3110S.gb/3013611‑3013671                                                           |   gcaagcTGGTTTTCCCCCCAGCCCCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:588525/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:679360/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:453888/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:552184/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:576591/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:578194/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:591381/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:620080/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:624048/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:315376/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:688475/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:738042/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:738157/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:869964/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:887568/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:922548/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:925612/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:956012/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1471635/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1149177/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1199622/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1223412/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1287104/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1296911/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1332228/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1363624/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:140621/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1103650/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1475054/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1491742/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1556080/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1563677/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1621283/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:1641075/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:261456/1‑61 (MQ=255) gcaagcTGGTTTTCCCCCCAGCACCAACAATAGAAATCACAGTGGGACGTTTCTGCGCGcc  >  1:314707/1‑61 (MQ=255)                                                           |   GCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACC  >  W3110S.gb/3013611‑3013671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑