Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,016,320	T→G	D535E (GAT→GAG)	ygfK →	predicted oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,016,320	0	T	G	100.0%	17.3 / NA	8	D535E (GAT→GAG)	ygfK	predicted oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/0);  total (8/0)

CTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACG  >  W3110S.gb/3016273‑3016334                                                |               cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:1080570/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:1199429/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:1544573/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:1548252/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:1628081/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:770962/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:808602/1‑62 (MQ=255) cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg  >  1:910444/1‑62 (MQ=255)                                                |               CTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACG  >  W3110S.gb/3016273‑3016334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑