Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,031,957 A→G A79A (GCA→GCG)  idi → isopentenyl diphosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,031,9570AG100.0% 315.2 / NA 108A79A (GCA→GCG) idiisopentenyl diphosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (108/0);  total (108/0)

Only 100 of 108 total aligned reads displayed.

GGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTT  >  W3110S.gb/3031922‑3031984
                                   |                           
gggCCCCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:758131/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCgccgttgcc             >  1:1305917/1‑52 (MQ=39)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCgccgtt                >  1:1409245/1‑49 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATg       >  1:1532728/1‑58 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGc    >  1:1321249/1‑61 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:507527/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:665232/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:649369/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:644588/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:636335/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:588521/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:567083/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:560696/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:54848/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:696453/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:497351/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:46271/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:448535/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:386695/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:365009/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:355307/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:287096/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:285686/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:274358/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:255099/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:247568/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:221374/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:871601/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:988459/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:988223/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:982412/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:973322/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:932936/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:928897/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:927925/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:927252/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:91554/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:912277/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:903016/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:675810/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:829033/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:814815/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:808088/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:796605/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:771573/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:766435/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:750769/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:733300/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:711666/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:208799/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:692238/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1184650/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1355974/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1348137/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1322535/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:131698/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1315793/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1301999/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1276705/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1262365/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1253961/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1235693/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1232441/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1213812/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1393179/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1180714/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1170898/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1163423/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1150856/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1124492/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1094511/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1084073/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1079951/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1048875/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1046876/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1041638/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1417611/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1022513/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:196386/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:191347/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:184292/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:182763/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:169478/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1643941/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1640261/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1620381/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1617358/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1615336/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:15934/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1586269/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1575456/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1564028/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1533100/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1524855/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1523326/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1508920/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:1445564/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCt   >  1:210282/1‑62 (MQ=255)
gggCACCCACAACTGGGAGAAAGCAACG‑AGAGGCGGTGATCCGCCGTTGCCGTTATGAGCtt  >  1:214630/1‑62 (MQ=255)
 ggCACCCACAACTGGGAGAAAGCAACGAAGAGGCGGTGATCCGCCGTTGCCGTTATGAGCtt  >  1:242598/1‑62 (MQ=255)
                                   |                           
GGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTT  >  W3110S.gb/3031922‑3031984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑