Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 352,544 C→T V205V (GTC→GTT)  prpE → predicted propionyl‑CoA synthetase with ATPase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb352,5440CT98.7% 216.1 / ‑5.6 78V205V (GTC→GTT) prpEpredicted propionyl‑CoA synthetase with ATPase domain
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (77/0);  total (78/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATC  >  W3110S.gb/352521‑352581
                       |                                     
gggCTGGCGATAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:396591/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCg                       >  1:531384/1‑40 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGAtt                    >  1:789011/1‑43 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTgcgc      >  1:1204384/1‑57 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTg         >  1:842134/1‑54 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCa    >  1:206259/1‑59 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:592583/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:757578/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:754787/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:75289/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:701078/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:675442/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:642421/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:612543/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:451585/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:57863/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:574565/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:566539/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:507248/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:501983/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:490677/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:441404/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:876672/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:995680/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:995206/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:990667/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:985127/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:938059/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:919615/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:907433/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:891189/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:780473/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:872222/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:858701/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:851504/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:847375/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:834874/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:81880/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:815107/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:810316/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:135335/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1003312/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1574783/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1536504/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1528365/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1517650/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1503975/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1414045/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1401631/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1380559/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1594912/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1345582/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1258488/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1240781/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1209525/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:11669/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1161238/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1055621/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1024993/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:317882/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:434491/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:432840/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:431328/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:428862/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:423413/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:396451/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:389087/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1598228/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:312863/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:290976/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:244491/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:240852/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:180760/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1631602/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1629739/1‑61 (MQ=255)
gggCTGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGACGAt                     >  1:715356/1‑42 (MQ=38)
gggCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:518185/1‑61 (MQ=255)
gggATGGCGAAAATGGCGCGCGTTAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATc  >  1:1230015/1‑61 (MQ=255)
                       |                                     
GGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATC  >  W3110S.gb/352521‑352581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑