Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,116,866 T→A T301T (ACA→ACT)  yghJ ← predicted inner membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,116,8660TA100.0% 319.1 / NA 111T301T (ACA→ACT) yghJpredicted inner membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/111);  total (0/111)

Only 100 of 111 total aligned reads displayed.

ACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAC  >  W3110S.gb/3116815‑3116876
                                                   |          
agcAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1230406/60‑1 (MQ=255)
aCCAAAGGAGATGGTTTCTCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1470143/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCTTTTTCGCCAGTTACGCCAc  <  1:1352252/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCTCCAGTTACGCCAc  <  1:488134/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:414786/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:607104/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:551155/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:530303/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:496673/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:470805/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:452039/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:450684/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:445734/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1006693/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:41391/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:359378/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:350216/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:300325/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:298398/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:295347/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:272149/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:259328/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:259223/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:247922/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:22769/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:227230/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:865112/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:987432/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:98055/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:979501/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:977868/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:972622/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:972106/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:956985/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:952010/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:945588/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:899620/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:895395/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:612028/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:851082/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:832416/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:819043/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:804377/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:795994/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:782004/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:772672/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:762974/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:691780/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:646801/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1176842/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1380453/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1368299/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1330197/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1318781/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1305023/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1294703/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1279052/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1274810/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1229862/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1211202/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1195942/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:219697/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1145448/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1136717/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:107369/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:106350/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1060247/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1048277/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1037691/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1029614/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1014660/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1008312/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1537206/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:226334/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:190552/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:17430/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1630099/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1626023/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:161248/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1597855/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1596574/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1558982/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1541814/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1422317/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1528409/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1523480/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1522953/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1516631/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1498609/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1475296/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:147181/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1460246/62‑1 (MQ=255)
aCCAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCAAGTTACGCCAc  <  1:899488/62‑1 (MQ=255)
 cctaaGGAGATGGGTTCGCCCCAGCTGAAGTTGAATTTGCCGGTTTCGCCAGTTACGCCAc  <  1:30071/58‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGTCGTTTTCGCCAGTTACGCCAc  <  1:457533/61‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1198922/61‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:689914/61‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:61780/61‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1019121/61‑1 (MQ=255)
 ccAAAGGAGATGGTTTCGCCCCAGCTGAAGTTGAATTTGCCGTTTTCGCCAGTTACGCCAc  <  1:1362527/61‑1 (MQ=255)
                                                   |          
ACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAC  >  W3110S.gb/3116815‑3116876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑