| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,148,896 | T→C | S193S (AGT→AGC) | yghA → | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,148,896 | 0 | T | C | 94.8% | 444.4 / 5.6 | 174 | S193S (AGT→AGC) | yghA | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base C (165/0); total (165/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-01 | |||||||||||
Only 100 of 174 total aligned reads displayed.
CCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACA > W3110S.gb/3148865‑3148945 | ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCAttt > 1:1312184/1‑40 (MQ=38)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTa > 1:560342/1‑41 (MQ=38)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTAc > 1:1625665/1‑58 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:973070/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:376955/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:708941/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:716328/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:189504/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:883057/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGaa > 1:117763/1‑61 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:609143/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:561362/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:586918/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:600329/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:470798/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:609708/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:625038/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:533233/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:529437/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:513791/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1003653/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:452416/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:424460/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:415493/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:406092/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:389157/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:343440/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:335498/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:851024/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:995722/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:969396/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:952917/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:92921/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:92288/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:920437/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:894332/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:881211/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:871953/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:858849/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:64723/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:831556/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:826670/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:812627/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:800738/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:7764/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:771991/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:737277/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:732342/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:679050/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:666240/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1187935/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:281456/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1443302/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1395528/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1358012/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1334314/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1323553/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:127806/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1273116/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1252653/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1199621/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1191604/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1460704/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1185366/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1166362/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1137434/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:111858/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1101064/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1071939/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1044808/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1040581/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:101785/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1005866/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1596397/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:257756/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:236676/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:219009/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:20057/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1642509/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1642297/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1615757/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1607896/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:160456/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1461061/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1579232/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1562995/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1556363/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1546596/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1498159/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1483321/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:148233/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1477035/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:1465305/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAGCCAAGCCAGCATTTACTGGACTATGCGGCTACGAAg > 1:35557/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATACCAG‑CAAGCCCGCATTTACTGGACTATGCGGCTACGAAgg > 1:669159/1‑62 (MQ=255)ccacTTCCTCAATCCAGGCATAACAGCCAAGCCCGCATTTACTGGACTATGCGGCTACGAAg > 1:426775/1‑62 (MQ=255)ccacTTACTCAATCCAGGCATACAAGCCAAGCCCGCAt > 1:1398644/1‑38 (MQ=21)ccaaTTCCTCAATCCAGGCATACCAGCCAAGCCCGCAtt > 1:479256/1‑39 (MQ=37) aTACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACa < 1:1317691/62‑1 (MQ=255) aTACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACa < 1:961861/62‑1 (MQ=255) | CCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACA > W3110S.gb/3148865‑3148945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |