Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,214,845	A→G	I101I (ATT→ATC)	yqjH ←	predicted siderophore interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,214,845	0	A	G	100.0%	133.7 / NA	47	I101I (ATT→ATC)	yqjH	predicted siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/0);  total (47/0)

TGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAC  >  W3110S.gb/3214831‑3214891               |                                               tGAATTAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1199108/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTGATGGCGTAGTTCGTc                      >  1:1557257/1‑41 (MQ=38) tGAATAAAGAAATCGATCGCCAGTTGATGGCGTAGTTCGTc                      >  1:696682/1‑41 (MQ=38) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAg                            >  1:1401633/1‑35 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGtt                          >  1:456634/1‑37 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATa                   >  1:193114/1‑44 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAg                >  1:879060/1‑47 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCTTATAGTCAc  >  1:1509061/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCa   >  1:440597/1‑60 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCa   >  1:130043/1‑60 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:714916/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:398558/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:435859/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:511422/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:552700/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:615160/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:697367/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:211360/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:718654/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:723615/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:760707/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:898424/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:90853/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:955415/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:958548/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:973595/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1515601/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1094114/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1137708/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1178460/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1191099/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1246569/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1255226/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1433875/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1440944/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1450673/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:366264/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1551093/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1582791/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:172280/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:180288/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1071644/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:220130/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:239445/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTAAc  >  1:1174111/1‑61 (MQ=255) tGAATAAAGAAATCGATCGCCAGTTCATGGAGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:462731/1‑61 (MQ=255) tGAAAAAAGAAATCGATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAc  >  1:1557532/1‑61 (MQ=255)               |                                               TGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAC  >  W3110S.gb/3214831‑3214891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑