Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,222,392 | T→C | N368N (AAT→AAC) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,222,392 | 0 | T | C | 99.5% | 550.2 / NA | 188 | N368N (AAT→AAC) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (0/187); minor base G (0/1); total (0/188) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 190 total aligned reads displayed.
GATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGA > W3110S.gb/3222366‑3222427 | gATCTCCAGTTGATGAAGCAGCACAAGATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1112979/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACTTCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:292652/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTTCGTACCGCTCACTACCCGAACGa < 1:346327/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:644983/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:73488/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:630124/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:611568/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:588280/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:5862/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:577295/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:572370/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:563402/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:547167/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:521296/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:477952/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:457512/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:443593/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:41350/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:412717/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:407624/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:396838/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:393536/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:370672/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:329307/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:309990/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:850/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:990380/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:97465/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:961755/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:955900/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:940852/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:939015/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:922192/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:908334/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:901203/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:891606/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:872837/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:670988/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:839927/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:836348/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:826764/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:821965/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:800378/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:78230/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:777924/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:773616/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:765374/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:749605/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:270519/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1147652/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1321411/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1302327/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1262373/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1260628/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1259021/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:124098/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1229284/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1201070/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1184418/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1176066/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1168776/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1164782/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:134662/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1143642/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1143240/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1142877/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1127703/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1123514/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1089364/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1079329/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1074493/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1058449/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1041264/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1022797/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1373494/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:218350/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:212056/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:197260/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:186749/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:169569/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1639277/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1633869/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1623977/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1617563/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:160093/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1568437/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1558767/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1557781/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1541647/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1522964/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1504921/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1484892/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1457693/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1435119/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1409143/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:239029/62‑1 (MQ=255) gATCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGAACCGCTCACTACCCGAACGa < 1:1425715/62‑1 (MQ=39) aTCTCCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:679600/61‑1 (MQ=255) tctcCAGTTGATGAAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:37870/60‑1 (MQ=255) tgatgaAGCAGCACAACATCAACTCCGTGCGTACCGCTCACTACCCGAACGa < 1:1011048/52‑1 (MQ=255) | GATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGA > W3110S.gb/3222366‑3222427 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |