| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,233,387 | T→C | intergenic (‑276/+8) | ygjN ← / ← ygjO | conserved hypothetical protein/predicted methyltransferase small domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,233,387 | 0 | T | C | 96.3% | 478.4 / 1.9 | 190 | intergenic (‑276/+8) | ygjN/ygjO | conserved hypothetical protein/predicted methyltransferase small domain |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base C (183/0); total (183/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
Only 100 of 191 total aligned reads displayed.
AGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCG > W3110S.gb/3233348‑3233420 | atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACcga > 1:949852/3‑54 (MQ=255)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:41307/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:570921/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:561616/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:552071/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:549997/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:507348/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:497673/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:495437/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:481258/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:475089/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:470438/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:440605/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:426931/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:597861/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:388357/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:383161/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:378447/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:376515/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:370243/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:370035/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:338732/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:32548/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:317634/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:28651/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:778866/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:994761/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:979606/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:962556/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:932305/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:924690/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:921223/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:899816/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:888135/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:880056/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:836557/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:806983/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1023421/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:768156/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:757121/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:746508/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:713518/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:693297/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:668038/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:659902/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:635481/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:634509/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:618302/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1235529/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:285027/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1427320/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1412537/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1411624/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1376312/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1370930/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1336327/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1318521/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1300145/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1293272/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1267987/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:123651/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1428145/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1232404/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1224323/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1188592/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1184282/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1165089/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1140810/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1124739/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:109308/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1068432/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1060172/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1000513/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1451065/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:276615/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:275220/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:265103/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:257279/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:241356/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:228604/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:20220/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:175576/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1651074/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1554138/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1434284/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1457540/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1473705/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1524609/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1535206/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1610182/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1568711/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:1579865/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:159304/3‑59 (MQ=25)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacg > 1:40966/3‑59 (MQ=255)atgCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacg > 1:1250863/3‑59 (MQ=255)atgCCGGATAAGGCGTTTAAGCCGCATCCGGCAGGTAACCCCCAATCTTACCGacgacgc > 1:300414/3‑59 (MQ=25) gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCg < 1:329869/61‑1 (MQ=255) gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCg < 1:9784/61‑1 (MQ=255) gCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCg < 1:1621611/61‑1 (MQ=255) cGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCg < 1:201099/60‑1 (MQ=255) | AGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCG > W3110S.gb/3233348‑3233420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |