Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,240,302	C→T	Q33Q (CAG→CAA)	ygjV ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,240,302	0	C	T	100.0%	274.1 / NA	96	Q33Q (CAG→CAA)	ygjV	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (96/0);  total (96/0)

AAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTC  >  W3110S.gb/3240265‑3240325                                      |                        aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAg             >  1:928232/1‑50 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCg           >  1:786084/1‑52 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTc     >  1:1438041/1‑58 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGt   >  1:77264/1‑60 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:627201/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:720422/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:694/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:692811/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:685942/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:675982/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:670173/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:641020/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:629976/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:62759/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:742715/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:625016/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:6125/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:577363/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:51354/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:510108/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:458404/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:44997/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:441292/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:429745/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:417291/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:403288/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:864457/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:985688/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:966614/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:932863/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:928929/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:921897/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:917874/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:917253/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:911767/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:908215/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:869877/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:735982/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:841690/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:838613/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:789068/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:788413/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:788083/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:78430/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:776305/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:769183/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:760556/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:401202/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1242552/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1397770/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1002055/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1328177/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1319649/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1311442/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1307183/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1297265/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1295590/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1282729/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:12656/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1263226/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1378400/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1207599/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1192703/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1168164/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1167583/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1153125/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1146973/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1113485/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1074517/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1036119/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1031557/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1587825/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:399131/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:341048/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:325949/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:285230/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:277512/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:274332/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:247642/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:226221/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1608225/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1588341/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1403246/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1568900/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1543121/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1489600/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1481825/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1458299/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1442152/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1423187/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1418864/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1418514/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1413245/1‑61 (MQ=255) aaaaaTGTACGCCAATAACGGCGCTATAGACCGAAAGTTGATTTTTGAAGCGACGTTCGTc  >  1:1263760/1‑61 (MQ=255) aaaaaTGTACGCCAAGACCGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:877478/1‑61 (MQ=255) aaaaaTGTACACCAATAACGGCGCTATAGACCGAAAGTTGCTTTTTGAAGCGACGTTCGTc  >  1:1183725/1‑61 (MQ=255)                                      |                        AAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTC  >  W3110S.gb/3240265‑3240325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑