Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,274,282 G→A L161L (CTG→TTG)  garP ← predicted (D)‑galactarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,274,2820GA100.0% 731.3 / NA 259L161L (CTG→TTG) garPpredicted (D)‑galactarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (259/0);  total (259/0)

Only 100 of 259 total aligned reads displayed.

AGCGGCGAAAAGAGCGCCAGCGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTACCA  >  W3110S.gb/3274263‑3274325
                   |                                           
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGATTTAAAGATGGCGTAGGCAGTAcc   >  1:1515325/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTaa                      >  1:846301/1‑43 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTa                       >  1:1578907/1‑42 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGAt                  >  1:643146/1‑47 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAg           >  1:1467344/1‑54 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCa        >  1:1246592/1‑57 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCa        >  1:299470/1‑57 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAg       >  1:1279422/1‑58 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTa     >  1:1594444/1‑60 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:451865/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:432633/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:445918/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:408154/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:469182/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:488748/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:502441/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:536561/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:418774/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:584476/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:38630/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:372738/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:364377/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:353744/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:346286/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:327686/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:304684/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:29215/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:27116/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:712655/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:969120/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:936557/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:930189/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:892332/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:888195/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:810380/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:80018/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:746402/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:739217/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:724034/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:716442/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:56982/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:69058/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:689655/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:66860/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:65356/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:635829/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:62366/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:618923/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:601900/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:245024/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:577974/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1154257/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1369548/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1000491/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1350439/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1344233/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:132865/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1307714/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1264493/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1250988/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1245333/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1206164/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:135735/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1148432/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1137683/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1117394/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1115610/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:11009/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1094698/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1088430/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1067484/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:106480/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1020662/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1391405/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:22354/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:215090/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:192248/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:18181/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1645709/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1638717/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1631738/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1601230/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1569207/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1560262/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:153648/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1511020/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1494464/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1480227/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1439668/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1434035/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1420676/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1398501/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1386082/1‑62 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAc    >  1:704873/1‑61 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAc    >  1:776096/1‑61 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAc    >  1:82815/1‑61 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAc    >  1:564098/1‑61 (MQ=255)
agcGGCGAAAAGAGCGCCAACGAGAAATATTGCGCCGAGTTAAAGATGCCGGAGGCAGTAcc   >  1:685451/1‑62 (MQ=255)
agcGGCGAAAAGAGCGACAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:119181/1‑62 (MQ=255)
agaggcgAAAAGAGAGCCAACGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTAcc   >  1:1530928/4‑62 (MQ=255)
                   |                                           
AGCGGCGAAAAGAGCGCCAGCGAGAAATATTGCGCCGAGTTAAAGATGGCGGAGGCAGTACCA  >  W3110S.gb/3274263‑3274325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑