Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,318,163	C→A	intergenic (‑19/‑329)	metY ← / → argG	tRNA‑Met/argininosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,318,163	0	C	A	100.0%	25.3 / NA	11	intergenic (‑19/‑329)	metY/argG	tRNA‑Met/argininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/11);  total (0/11)

GCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAA  >  W3110S.gb/3318121‑3318182                                           |                    gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:1035025/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:1096986/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:115179/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:1266975/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:1281858/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:133736/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:1379308/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:197770/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:239882/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:380538/62‑1 (MQ=255) gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa  <  1:396270/62‑1 (MQ=255)                                           |                    GCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAA  >  W3110S.gb/3318121‑3318182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑