Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,326,259	G→T	Q178K (CAG→AAG)	ftsH ←	protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,326,259	0	G	T	76.7%	42.5 / 12.5	30	Q178K (CAG→AAG)	ftsH	protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base G (0/7);  new base T (23/0);  total (23/7)
Fisher's exact test for biased strand distribution p-value = 4.91e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGA  >  W3110S.gb/3326227‑3326281                                 |                       gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:1130513/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:460712/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:856204/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:1365183/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:787806/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:585946/48‑1 (MQ=255) gACGCCTTTAGGGATCTTACCGCCGAGTTTCTGGAAGCggctcggctc         <  1:166649/48‑1 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAg   >  1:1315475/1‑47 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:759973/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:388682/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:467168/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:526287/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:543342/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:545344/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:286490/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:932788/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:997790/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:318045/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1069132/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:266514/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:211004/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:209615/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1605470/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1487010/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1454489/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1407644/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1249473/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1150628/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGCGGCTCGGCTCGCGCAGa  >  1:1092405/1‑48 (MQ=255)        ttCGGGATCTTACCGCCGAGTTTCTTGAAGAGGCTCGGCTCGCGCAGa  >  1:437569/1‑48 (MQ=255)                                 |                       GACGCCTTTCGGGATCTTACCGCCGAGTTTCTGGAAGCGGCTCGGCTCGCGCAGA  >  W3110S.gb/3326227‑3326281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑