Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,338,549 G→A F185F (TTC→TTT)  yrbE ← predicted toluene transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,338,5490GA100.0% 257.3 / NA 90F185F (TTC→TTT) yrbEpredicted toluene transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/90);  total (0/90)

CGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCG  >  W3110S.gb/3338524‑3338585
                         |                                    
cGACTGCATTTTGCATTTCCGACCAAAATAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:711617/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTTACGCCg  <  1:986990/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGcag  <  1:187568/62‑3 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:484462/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:569239/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:559982/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:533660/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:530914/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:530507/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:521210/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:505642/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:495412/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:487040/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:578403/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:466992/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:380485/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:363982/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:332349/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:329765/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:250670/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:244369/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:244357/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1010890/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:980703/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:94837/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:911707/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:910611/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:906612/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:881165/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:857179/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:80003/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:76783/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:723607/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:949124/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:669865/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:641410/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:632112/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:615756/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:613778/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:603752/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:597040/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:595527/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:580841/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1298129/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:176805/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1447272/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1437040/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1416504/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1393250/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1391/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1358602/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:132143/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1314200/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1299255/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:146480/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1269001/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1233451/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1169990/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1135500/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1135187/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1110189/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1102324/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1097150/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1027859/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1005388/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1547945/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:168341/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1650848/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1645539/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:159933/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1599172/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1591930/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1587617/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1556908/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1553525/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1533225/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1524147/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1517080/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1503183/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1499227/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1476792/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1469578/62‑1 (MQ=255)
cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1467739/62‑1 (MQ=255)
 gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1301110/61‑1 (MQ=255)
 gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:574333/61‑1 (MQ=255)
 gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:287165/61‑1 (MQ=255)
 gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1478747/61‑1 (MQ=255)
 gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:1110092/61‑1 (MQ=255)
    ggCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg  <  1:645302/58‑1 (MQ=255)
          ttGCATTGCCGACCAAATGAACCCGCTATCAATGCCTTTCCTACTGACGCCg  <  1:214235/52‑1 (MQ=255)
                         |                                    
CGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCG  >  W3110S.gb/3338524‑3338585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑