| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,495,020 | T→G | A187A (GCT→GCG) | ptsA → | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,495,020 | 0 | T | G | 99.2% | 688.7 / ‑5.0 | 239 | A187A (GCT→GCG) | ptsA | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (0/236); minor base A (0/2); total (0/239) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
Only 100 of 239 total aligned reads displayed.
AACATTGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCTCACCG > W3110S.gb/3494964‑3495025 | aaCATAGAGTTTGGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:379342/62‑1 (MQ=255)aaCATAGAGTTTCTTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1510556/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCTATTCTGGAAGCGCACCg < 1:1553377/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:64412/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:674733/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:640320/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:623186/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:59229/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:569616/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:533928/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:520283/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:500710/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:490195/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:481470/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:473403/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:469553/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:445603/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:400031/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:394121/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:368753/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:353049/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:29864/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:278814/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:260731/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:241314/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:860500/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:988676/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:987248/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:979532/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:965900/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:957175/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:953460/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:916035/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:898239/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:884751/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:874849/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:865938/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:864474/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:23658/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:850371/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:841952/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:815313/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:805653/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:782543/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:778185/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:759087/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:738922/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:72164/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:707029/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:690040/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1200881/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:136828/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:136624/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1319798/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1316359/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:131284/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1295045/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1291201/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1285466/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1264943/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1245065/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1228815/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:236176/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1175565/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1160994/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1140305/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1128667/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1122054/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1102137/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1097878/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1081939/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1061064/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1046321/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1020465/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1416331/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1005573/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:208693/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:185936/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1627153/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1623495/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1615385/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1603470/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1599883/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1584711/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1573179/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1564923/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1548358/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1531331/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1505982/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1473590/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1456834/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1435204/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1420269/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1411308/62‑1 (MQ=255)aaCATAGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCACACCg < 1:1489381/62‑1 (MQ=255) aGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:548813/55‑1 (MQ=255) ggATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1485545/42‑1 (MQ=255) aTAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1011482/40‑1 (MQ=255) tAGCGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:1335967/39‑1 (MQ=255) cGACGGTGCTACCAGCGCGATTCTGGAAGCGCACCg < 1:269232/36‑1 (MQ=255) | AACATTGAGTTTCGTCTGCTGGATAGCGACGGTGCTACCAGCGCGATTCTGGAAGCTCACCG > W3110S.gb/3494964‑3495025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |