Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,551,998 T→C C380C (TGT→TGC)  fdoG → formate dehydrogenase‑O, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,551,9980TC99.4% 386.0 / ‑5.4 174C380C (TGT→TGC) fdoGformate dehydrogenase‑O, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (173/0);  minor base A (1/0);  total (174/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 174 total aligned reads displayed.

GAAACAGCACGTTTCCCGCTACACGCCAGATGTGGTTGAAAACATCTGTGGTACGCCAAAAGAC  >  W3110S.gb/3551950‑3552013
                                                |               
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACTCCAAAAg    >  1:993388/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGTCAAAAg    >  1:171116/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:592098/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:695139/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:1276856/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:1586948/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:1097646/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:850533/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCaaaa     >  1:878097/1‑61 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCa        >  1:1214036/1‑58 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:460824/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:550465/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:214580/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:542717/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:528383/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:52001/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:503870/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:294007/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:496349/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:480923/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:350290/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:310571/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:448283/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:437644/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:421792/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:265413/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:323688/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:380587/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:338413/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:350805/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:797357/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:992540/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:990608/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:978952/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:955924/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:950999/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:926123/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:920516/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:862823/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:84089/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:808851/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:567947/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:784821/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:731037/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:726803/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:720583/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:703847/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:679004/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:64729/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:641281/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:614310/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:572849/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:118995/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:221434/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1362640/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1324042/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1300160/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:128863/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1282912/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1250162/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1238767/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1217392/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1205079/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1199841/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1371026/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:118617/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1139549/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:111819/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1113580/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:109337/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1076359/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1052596/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1050106/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1046514/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1029145/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:153834/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:102269/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:211058/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:191891/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:169544/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1630499/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1599951/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1594036/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1578092/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1561899/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1550608/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:137156/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1514632/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1505799/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1499534/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1496052/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1459320/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1439370/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1428569/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:141635/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:1406675/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:139560/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGCCAGATCTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:404332/1‑62 (MQ=17)
gAAACAGCACGTTTCCCGTTATACGACAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAg    >  1:858275/1‑62 (MQ=17)
  aaCAGCACGTTTCCCGTTATACGCCAGATGTGGTTGAAAACATCTGCGGTACGCCAAAAGAc  >  1:1327665/1‑62 (MQ=17)
                                                |               
GAAACAGCACGTTTCCCGCTACACGCCAGATGTGGTTGAAAACATCTGTGGTACGCCAAAAGAC  >  W3110S.gb/3551950‑3552013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑