Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,609,702	C→T	E151E (GAG→GAA)	fre ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,609,702	0	C	T	100.0%	43.0 / NA	17	E151E (GAG→GAA)	fre	flavin reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/17);  total (0/17)

GACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCA  >  W3110S.gb/3609671‑3609732                                |                               gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:532245/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:987338/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:95917/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:947983/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:83575/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:70641/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:702167/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:641651/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:533871/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:1017824/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:528798/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:470150/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:1476863/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:1312667/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:1259112/62‑1 (MQ=255) gACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:1250762/62‑1 (MQ=255) gACCAGGATGCTT‑AACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCa  <  1:133992/61‑1 (MQ=255)                                |                               GACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCA  >  W3110S.gb/3609671‑3609732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑