Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,628,930 T→A intergenic (‑6/+58) rhtC ← / ← recQ threonine efflux system/ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,628,9300TA100.0% 551.0 / NA 190intergenic (‑6/+58)rhtC/recQthreonine efflux system/ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/190);  total (0/190)

Only 100 of 190 total aligned reads displayed.

ACATCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAG  >  W3110S.gb/3628915‑3628956
               |                          
aCATCAACATACATTATACTCGCGGGGGAAACGTTTACGGAg  <  1:1522814/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCTGGGGAAACGTTTACGGAg  <  1:930227/42‑1 (MQ=38)
aCATCAACATACATTAGACTCGCGTGGGAAACGTTTACGGAg  <  1:264347/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGat  <  1:951137/42‑2 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGa   <  1:1380434/41‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:449753/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:60527/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:597879/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:579197/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:572322/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:563981/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:54454/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:541147/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:519859/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:500739/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:472833/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:465649/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:666691/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:439239/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:43128/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:420335/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:409782/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:385041/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:367034/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:356697/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:336357/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:322262/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:808300/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:989063/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:9828/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:973411/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:939678/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:906189/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:901827/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:856872/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:85258/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:83709/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:819731/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:813479/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:628663/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:79856/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:784101/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:770640/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:749337/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:731802/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:723851/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:710815/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:705647/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:685190/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:315105/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:654354/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1172244/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1405313/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1398846/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1350862/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1343019/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:133245/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1322605/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1318244/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1290961/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1256807/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1213590/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1195716/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1418631/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1163683/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1158394/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1136516/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1131936/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:112645/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:111870/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1096894/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1074342/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1069484/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1052897/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1003926/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1595825/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1000785/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:302778/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:28847/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:275911/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:22852/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:199629/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:185805/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:182425/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1632834/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1630662/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1617939/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:317023/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1573044/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1508631/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1493943/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1489086/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1480137/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1454692/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1450441/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:144353/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1427858/42‑1 (MQ=255)
aCATCAACATACATTAGACTCGCGGGGGAAACGTCTACGGAg  <  1:1584802/42‑1 (MQ=255)
 cATCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:1227618/41‑1 (MQ=255)
  aTCAACATACATTAGACTCGCGGGGGAAACGTTTACGGAg  <  1:375017/40‑1 (MQ=255)
               |                          
ACATCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAG  >  W3110S.gb/3628915‑3628956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑