Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,643,603 A→G Y642Y (TAT→TAC)  cyaA ← adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,643,6030AG100.0% 371.5 / NA 127Y642Y (TAT→TAC) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (127/0);  total (127/0)

Only 100 of 183 total aligned reads displayed.

TACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCTATAACAGAAGACTTC  >  W3110S.gb/3643545‑3643617
                                                          |              
taaacTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:600919/58‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1499039/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1298511/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1317686/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:730/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1408354/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1443922/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:674192/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:671435/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:511174/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:537409/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1310530/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:151034/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:560328/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1524271/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1532347/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1613730/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:610800/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1595766/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:572482/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1087925/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1127606/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:366012/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:407244/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1188305/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:109628/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1093395/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:300457/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1052111/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1239816/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1037591/61‑1 (MQ=255)
tACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:1282439/61‑1 (MQ=255)
 acacTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCTCGCAGATGCTGGCtata              <  1:924335/60‑1 (MQ=255)
 acacTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCtata              <  1:241358/60‑1 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGCTGCTGGCTGTAGCAGAAGACTTc  >  1:911652/1‑59 (MQ=38)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:212660/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:56569/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:427840/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:457159/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:462659/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:510966/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:503696/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:475819/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:390899/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:495143/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:775963/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:9938/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:992949/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:940602/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:917255/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:908182/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:868476/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:860933/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:828785/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:587552/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:770426/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:693838/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:683258/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:65384/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:647596/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:631535/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:617614/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1224006/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1483946/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1461865/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1447386/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1430247/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1358691/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1335585/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1318687/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1268326/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1244650/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1495515/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1219637/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1191252/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:117721/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1163222/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1157390/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1148140/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1101086/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1018922/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:189792/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:375880/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:365193/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:334794/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:312219/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:279773/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:244969/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:226005/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1001536/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:205278/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:386525/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:187374/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1602144/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:159979/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1583965/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1577254/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1566334/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1553824/1‑59 (MQ=255)
              aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1517272/1‑59 (MQ=255)
                                                          |              
TACACTCAGAAACCAGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCTATAACAGAAGACTTC  >  W3110S.gb/3643545‑3643617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑