BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,739,327	C→T	V195I (GTT→ATT)	yieH ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,739,327	0	C	T	100.0%	337.5 / NA	118	V195I (GTT→ATT)	yieH	predicted hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (48/70); total (48/70)

Only 100 of 118 total aligned reads displayed.

AAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCT > W3110S.gb/3739294‑3739376

aaaGATGGGTAAAGGTGGTGTCTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1062461/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGGTCGGCTTATTGTGCGGGTCGgcgc                         >  1:1195718/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1196203/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:142479/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1463670/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1569172/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1631542/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1634340/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:19796/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1245458/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1223156/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:486054/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1202184/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:521846/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1170365/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1167927/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:955365/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1022280/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:922414/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:819647/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:717114/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1069930/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:1030698/1‑60 (MQ=255)

aaaGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGgcgc                         >  1:764404/1‑60 (MQ=255)

             ggttgtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:567753/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATtgt                                     >  1:27446/1‑35 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:223412/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:274750/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:490307/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:896168/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:700290/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1523602/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1522551/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1495315/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1015332/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1436880/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:575360/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1080922/1‑61 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGGCGGCGCAGAAGTAGAACACtt          <  1:284024/62‑1 (MQ=255)

             ggtggtGACTTTCGGGTGAATGATCCGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACt           >  1:1028950/1‑61 (MQ=255)

                    aCTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt          <  1:1307901/55‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:626698/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:708075/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:615335/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:598390/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1119319/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1184381/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:517234/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:515536/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1213683/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:455513/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:418298/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:407029/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:406375/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:108514/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:701036/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:379221/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:734137/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1034485/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:78232/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:828621/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:85798/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:858321/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:892104/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1027430/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:914597/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1022657/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1004561/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1283019/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:139552/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1423288/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1376284/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1349065/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1451934/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1459760/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1343680/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:150776/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1518868/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1297484/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1560187/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1567348/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1285033/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1625113/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1376375/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1280766/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:175930/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1252228/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:240983/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:261972/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1250061/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:124209/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:274966/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:340476/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:358598/61‑1 (MQ=255)

                      tttCGGGTGAATGATCGGCTTATTGTGCGCGTCGGCGCACAAGTAGAACACTTCCATACCt  <  1:255219/61‑1 (MQ=255)

                      tttCGGGGGAATGATCGGCTTATTGTGCGGGGCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1251210/61‑1 (MQ=255)

                      tttCGAGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1293057/61‑1 (MQ=255)

                      cttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1204707/60‑1 (MQ=255)

                       ttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:592469/60‑1 (MQ=255)

                           ggTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCt  <  1:1150795/56‑1 (MQ=255)

AAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCT > W3110S.gb/3739294‑3739376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`