Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,742,233 T→C E194E (GAA→GAG)  yieE ← predicted phosphopantetheinyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,742,2330TC100.0% 651.8 / NA 220E194E (GAA→GAG) yieEpredicted phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/220);  total (0/220)

Only 100 of 220 total aligned reads displayed.

GACACTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCTTCCGCGTC  >  W3110S.gb/3742179‑3742241
                                                      |        
gACACTGAGCTTTTCAATCGTGGGCGTGACC‑CCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:275742/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCTTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:347756/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGAGCACACCAGCACGTCCTCCGCGTc  <  1:518407/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:416032/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:595226/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:591803/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:580691/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:574486/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:566214/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:531286/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:492840/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:489614/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:457529/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:44586/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:424463/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:603612/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:372651/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:334524/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:324198/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:311174/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:27306/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:250244/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:229689/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1008736/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:199078/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:803858/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:994587/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:983255/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:979333/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:957478/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:944188/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:935421/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:897345/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:883955/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:865280/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:860204/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:820852/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:213291/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:789207/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:787079/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:762394/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:756639/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:69638/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:693062/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:684302/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:661201/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:640164/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:628716/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:605780/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1194450/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:188736/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1347689/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1340410/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1331903/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1292629/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1277712/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1271630/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1252101/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1235226/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1216483/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1204456/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1199146/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1368951/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1168077/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1166326/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:111790/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1113369/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1101797/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1084157/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1068308/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1060321/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1046134/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1036838/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1017168/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1514973/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:165150/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1644361/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1630516/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1609568/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1605688/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1583703/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1578860/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1576657/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1560059/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1532780/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1528416/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1369791/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1506093/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1482367/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1479387/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1472317/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1465320/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:145990/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1416320/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1397669/62‑1 (MQ=255)
 acacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1383381/62‑1 (MQ=255)
  cacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:342980/61‑1 (MQ=255)
  cacTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:1352436/61‑1 (MQ=255)
     tGAGCTTTTCAATCGTGGGCGTGACCGCCACTGACCACACCAGCACGTCCTCCGCGTc  <  1:501181/58‑1 (MQ=255)
                        cGTGACCGCCACGGACCACACCAGCACGTCCTCCGCGTc  <  1:646970/39‑1 (MQ=255)
                                                      |        
GACACTGAGCTTTTCAATCGTGGGCGTGACCGCCACGGACCACACCAGCACGTCTTCCGCGTC  >  W3110S.gb/3742179‑3742241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑