Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 448,438 G→A F476F (TTC→TTT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb448,4380GA100.0% 308.1 / NA 108F476F (TTC→TTT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (108/0);  total (108/0)

Only 100 of 108 total aligned reads displayed.

AACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCG  >  W3110S.gb/448419‑448480
                   |                                          
aaCGACGGGTCTTCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:385593/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATAc                             >  1:834275/1‑35 (MQ=25)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATTAAGGCAACGAAGAAGCCg  >  1:1538425/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAg                 >  1:296725/1‑47 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAg                 >  1:828921/1‑47 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGGAACGAAGAAGCCg  >  1:669991/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAAc            >  1:1514372/1‑52 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa      >  1:1511112/1‑58 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaa         >  1:972067/1‑55 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcct  >  1:655155/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcc   >  1:402014/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcc   >  1:18582/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcc   >  1:159874/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcc   >  1:1179013/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGcc   >  1:811888/1‑61 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:951777/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:526242/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:918237/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:52173/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:50491/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:927820/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:504114/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:487053/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:485176/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:473433/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:55403/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:463769/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:456475/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:445770/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:439819/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:433680/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:407859/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:956102/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:403746/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:382903/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:656829/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:794448/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:76910/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:744076/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:736558/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:73339/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:703653/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:700947/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:697786/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:682479/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:536538/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:819488/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:829021/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:65275/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:58405/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:866926/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:571904/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:564932/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:806162/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1225637/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:397719/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1454398/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1428227/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1372587/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1371425/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1340566/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1326945/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1309088/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1306171/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1301134/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1236832/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1456669/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1209706/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1203093/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1194614/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1187653/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1130914/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:112035/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1119644/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1094353/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1091852/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1033001/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:186097/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:38733/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1025812/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:331560/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:324371/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:31238/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:301852/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:298904/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:295639/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:248096/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:212583/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1471423/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:181638/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:173886/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1593604/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1583144/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1581369/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1562085/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1561453/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1513866/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1511632/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCCCAACGCATACAGAGGCATAAAGGCAACGAAGAAGCCg  >  1:416147/1‑62 (MQ=255)
aaCGACGGGTCATCCCCATAAAGCACAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCg  >  1:1321203/1‑62 (MQ=255)
                   |                                          
AACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCG  >  W3110S.gb/448419‑448480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑