| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,750,201 | G→A | T96T (ACC→ACT) | tnaA ← | tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,750,201 | 0 | G | A | 83.6% | 460.8 / 69.5 | 250 | T96T (ACC→ACT) | tnaA | tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (41/0); new base A (209/0); total (250/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-03 | |||||||||||
Only 100 of 250 total aligned reads displayed.
AATCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCT > W3110S.gb/3750147‑3750240 | aaTCAGTACCGGAATATAGATTTGCTCTGCGTCACGGCCCTGGTGAGTCGGAATGgtgt > 1:977152/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:55229/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1260531/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:540178/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:789642/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:217571/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:806330/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:857363/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:866707/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1334478/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1407148/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:110434/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1593824/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1543635/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1071102/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:1473906/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtgt > 1:585917/1‑59 (MQ=255)aaTCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGgtg > 1:1238397/1‑58 (MQ=255) ggTTAGTCGGAACAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:828876/1‑53 (MQ=37) ggTGAGTCGGAATAGTGTATTGTTAACCAAAGATATTTTTCACTGACTCGGCt > 1:609885/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGa > 1:441936/1‑46 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:51162/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:486475/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:481005/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:460316/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:557620/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:559562/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:563293/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:455198/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:579171/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:414495/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:396361/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:262842/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:361328/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:740789/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:99336/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:984198/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:966786/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:950739/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:939492/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:93334/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:889723/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:801208/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:763067/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:612812/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:736745/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:703388/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:698819/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:681526/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:663115/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:646769/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:639741/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:633441/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:628692/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1192925/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:354732/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1389373/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1386614/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1380877/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1324155/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1281899/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1270932/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1249435/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1234358/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:121014/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1205638/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:142991/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1182791/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:114417/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1130534/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1128266/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1116883/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1094793/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1087396/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1046348/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1040833/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:101622/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:175109/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:338661/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:324774/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:303002/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1003642/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:241452/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:228258/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:224742/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:198992/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:187170/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1440740/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1640887/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1617650/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1613824/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1601022/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1555686/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:153468/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1528249/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1498870/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1488862/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCt > 1:1454182/1‑53 (MQ=255) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATATATTTCACTGACTCGGCt > 1:427655/1‑53 (MQ=39) ggTGAGTCGGAATAGTGTATTGATAACCAAAGATAGTTTTCACTGACTCGGCt > 1:847802/1‑53 (MQ=39) | AATCAGTACCGGAATATAGATTTGCTCTGCGCCACGGCCCTGGTGAGTCGGAATGGTGTATTGATAACCAAAGATATTTTTCACTGACTCGGCT > W3110S.gb/3750147‑3750240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |