Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,820,897 | G→T | A551S (GCT→TCT) | ligB → | DNA ligase, NAD(+)‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,820,897 | 0 | G | T | 99.3% | 380.3 / NA | 140 | A551S (GCT→TCT) | ligB | DNA ligase, NAD(+)‑dependent |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (0/139); total (0/140) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 140 total aligned reads displayed.
TTATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGA > W3110S.gb/3820847‑3820909 | ttATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCcagcag < 1:72594/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:414611/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:613245/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:570530/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:567803/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:562079/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:53526/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:525236/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:507476/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:456855/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:45470/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:419030/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:418058/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:654411/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:414436/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:411603/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:386421/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:373388/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:349414/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:345797/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:320091/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:302405/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:302253/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:261951/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1016706/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:79664/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:97353/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:955988/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:922880/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:917004/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:893506/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:876183/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:859064/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:8375/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:834925/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:813164/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:805718/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:644644/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:796546/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:791557/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:762381/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:739303/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:734120/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:718788/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:692257/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:682226/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:672744/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:66992/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:260754/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1270068/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1413091/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1380042/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1367256/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1355771/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1338303/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1337990/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1334502/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1327699/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1317255/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:129291/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1271567/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:248652/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1243345/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:123551/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1217280/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1217084/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1198295/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1172062/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1155239/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1143819/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:109066/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1077939/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:103751/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1441899/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:249517/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:230883/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:208975/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:173763/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1643713/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1629143/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1620774/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1600533/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1593341/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1553965/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1432318/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1465446/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1467714/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1470610/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1534972/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1542034/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1547858/62‑1 (MQ=255) tATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGa < 1:1073855/62‑1 (MQ=255) aTTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:171244/61‑1 (MQ=255) aTTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1182790/61‑1 (MQ=255) aTTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:912737/61‑1 (MQ=255) aTTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1458204/61‑1 (MQ=255) gAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:101692/58‑1 (MQ=255) aggaaAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:262318/51‑1 (MQ=255) aaaTCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:215274/39‑1 (MQ=255) aTCAAGAAGCTGGGCAGTTGGCTGTCTGCCCAGCAGa < 1:1569387/37‑1 (MQ=255) | TTATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGA > W3110S.gb/3820847‑3820909 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |