| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,023,367 | T→A | L12L (CTA→CTT) | nikD ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,023,367 | 0 | T | A | 82.7% | 221.4 / 37.6 | 98 | L12L (CTA→CTT) | nikD | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/17); new base A (81/0); total (81/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
GCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAT > W3110S.gb/4023309‑4023402 | gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:259793/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:5043/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:483633/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:483121/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:461761/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:441829/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:441566/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:383011/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:286959/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:274712/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:260082/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:51054/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:244124/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:240549/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:240231/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:231414/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:222287/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:207655/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:207653/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:198588/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:195783/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:183264/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:726584/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:973664/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:951622/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:925663/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:906930/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:873054/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:824213/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:799012/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:786872/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:773983/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:747513/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1004250/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:704856/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:703716/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:621218/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:61823/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:5889/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:562248/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:554618/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:551800/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:534892/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1154610/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1303657/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1290562/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1276202/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1267931/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1264126/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:125910/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1257704/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1249943/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1236880/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1187282/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:173313/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1144053/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1112824/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1074495/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:106433/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1056564/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1049094/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:104418/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1017285/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1004725/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1000941/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1343518/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1590467/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1590005/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1559072/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:159929/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:162223/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1644271/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1646980/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1456356/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1442334/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1434017/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1372881/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:180208/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1315066/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1372669/1‑61 (MQ=255)gCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGAAg > 1:1347215/1‑61 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:96356/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:186440/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:1037867/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:218945/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:1069261/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:992734/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:1575921/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:678846/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:673387/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:651586/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:1490764/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:618583/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:1205340/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:617268/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:614915/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:318981/62‑1 (MQ=255) ccGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAt < 1:319832/62‑1 (MQ=255) | GCACGCGCCCGCGTTGCAGGGTTAACGATACACCGTGTACCAGCGGCTGCGCGGCCTGTAGCGCGATATTACGTAGTTCAATCTGTTGCGGCAT > W3110S.gb/4023309‑4023402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |