Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,046,306 G→A E32E (GAG→GAA)  livG → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,046,3060GA98.7% 214.0 / ‑5.9 77E32E (GAG→GAA) livGleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (76/0);  minor base T (1/0);  total (77/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACC  >  W3110S.gb/4046283‑4046345
                       |                                       
aaTCTTGAACTGTACCCGCAGGATATCGTCTCGTTAATCGGcc                      >  1:1070886/1‑43 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCt                    >  1:1346514/1‑45 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGaaaaa    >  1:1599983/1‑61 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGaaaaa    >  1:1501119/1‑61 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:523673/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:185154/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:520083/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:517909/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:507075/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:454798/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:409788/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:386846/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:352073/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:347417/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:344089/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:322411/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:2679/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:249464/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:227715/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:208732/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:984880/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:792386/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1017992/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:954470/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:944078/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:93607/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:91688/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:891116/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:891019/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:86149/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:82328/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:541689/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:79191/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:79133/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:773000/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:765509/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:678222/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:677861/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:645114/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:558069/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1225424/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1354974/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1352949/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1345271/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:130938/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1288734/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1281989/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1276383/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:126593/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1368734/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1223868/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1220305/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1200235/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1198769/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1155759/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1133061/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1125953/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1120125/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1489251/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:171121/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:167747/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1630473/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1614529/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1562930/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:152052/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:173255/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1483755/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1480473/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1442772/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1436547/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1406259/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1382676/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1379418/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGGCCATAACGGTGCCGGAAAAAc   >  1:242637/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGG‑CCTAACGGTGCCGGAAAAAcc  >  1:64197/1‑62 (MQ=255)
aaTCTTGAACTGTACCCGCAGGAAATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAAc   >  1:1629852/1‑62 (MQ=255)
aaTCTTGAACTGTACCAGCAGGAAATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc   >  1:1637438/1‑62 (MQ=255)
                       |                                       
AATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACC  >  W3110S.gb/4046283‑4046345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑