Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,233,675 T→C A20A (GCA→GCG)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,233,6750TC100.0% 397.4 / NA 135A20A (GCA→GCG) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/135);  total (0/135)

Only 100 of 135 total aligned reads displayed.

AAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGT  >  W3110S.gb/4233620‑4233681
                                                       |      
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:459475/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:712616/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:659254/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:653229/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:636173/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:627886/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:57357/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:566406/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:557495/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:545981/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:545738/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:530345/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:465404/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:728923/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:4397/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:413519/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:381856/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:350222/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:34579/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:337208/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:321128/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:318546/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:281327/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1018996/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:244877/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:83369/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:967791/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:966318/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:937748/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:931406/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:929905/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:926204/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:921001/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:894242/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:885651/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:882973/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:867379/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:277873/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:833496/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:833262/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:823844/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:822028/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:799281/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:78764/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:783514/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:774421/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:751533/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:744588/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:738740/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1144211/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1378896/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1333279/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1295032/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:128513/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1283885/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1261455/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1251322/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1197135/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1180977/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1171294/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1153849/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:219420/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1112836/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1109334/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1106182/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1104144/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1101481/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1093663/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1074632/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1072013/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:104261/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1037286/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:102298/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1520275/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1590802/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1650190/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1583550/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1582241/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1570234/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1560906/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1551213/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1549536/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1538394/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1498805/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:165303/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:17353/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1435441/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1400981/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1398459/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:215390/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1393237/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1379773/62‑1 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCGTgg  <  1:700497/62‑2 (MQ=255)
aaGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATAAGCGGCAGCGCgtgt  <  1:1025222/62‑1 (MQ=255)
 aGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:347536/61‑1 (MQ=255)
 aGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1487191/61‑1 (MQ=255)
 aGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:561468/61‑1 (MQ=255)
 aGGAATAAATACCGCAGAACGGCGACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1333204/61‑1 (MQ=255)
 aGGAATAAATACCGCAGAACGGCGACCCTGCAAATGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:223703/61‑1 (MQ=255)
                   acggcgACCCTGCAACTGTTCAGCAATCAGCGGCAGCGCgtgt  <  1:1037408/42‑1 (MQ=25)
                                                       |      
AAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGT  >  W3110S.gb/4233620‑4233681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑