Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,251,001	C→G	A209G (GCC→GGC)	malK →	fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,251,001	0	C	G	100.0%	17.1 / NA	13	A209G (GCC→GGC)	malK	fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/13);  total (0/13)

CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG  >  W3110S.gb/4250948‑4251009                                                      |         ccACGACCAGGTCGAGGCGATGACCCTGGCTGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:688307/62‑1 (MQ=16) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:108632/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1309460/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1427253/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1604304/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:397813/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:499759/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:629107/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:673187/62‑1 (MQ=12) ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:852803/62‑1 (MQ=12)  cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:390714/61‑1 (MQ=12)  cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:806145/61‑1 (MQ=12)   aCGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:417434/60‑1 (MQ=12)                                                      |         CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG  >  W3110S.gb/4250948‑4251009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑