Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,251,005 T→G G210G (GGT→GGG malK → fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,251,0050TG100.0% 17.4 / NA 13G210G (GGT→GGGmalKfused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/13);  total (0/13)

CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG  >  W3110S.gb/4250948‑4251009
                                                         |    
ccACGACCAGGTCGAGGCGATGACCCTGGCTGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:688307/62‑1 (MQ=16)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:108632/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1309460/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1427253/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:1604304/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:397813/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:499759/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:629107/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:673187/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:852803/62‑1 (MQ=12)
 cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:390714/61‑1 (MQ=12)
 cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:806145/61‑1 (MQ=12)
  aCGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg  <  1:417434/60‑1 (MQ=12)
                                                         |    
CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG  >  W3110S.gb/4250948‑4251009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: