Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,298,525	T→C	I151I (ATT→ATC)	gltP →	glutamate/aspartate:proton symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,298,525	0	T	C	100.0%	262.3 / NA	90	I151I (ATT→ATC)	gltP	glutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/90);  total (0/90)

CACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGA  >  W3110S.gb/4298481‑4298541                                             |                 cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:256157/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:550367/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:537658/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:48834/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:426721/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:412786/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:386321/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:341750/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:337672/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:277898/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:558249/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:248808/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:247037/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:184207/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1637894/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1614116/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1610678/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1593478/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1590954/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1569374/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:870060/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:99268/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:973518/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:972727/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:966453/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:944615/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:943408/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:925746/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:914532/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:914116/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1009643/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:860059/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:780071/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:749073/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:707911/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:694497/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:667439/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:663900/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:639269/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:637571/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1225016/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1411656/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1405513/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1555540/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1384447/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1330430/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1302508/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1289636/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1281580/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1274917/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1387300/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1217586/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1206450/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:116425/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1148834/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1100370/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1090892/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1059857/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1038376/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1002499/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:15132/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1550799/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1546534/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1417310/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1418618/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1545048/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1428918/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1542179/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1431741/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1541490/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1472632/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1475173/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1488541/61‑1 (MQ=255) cACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1497145/61‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:916239/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:871985/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1583307/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:480392/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:867113/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:213525/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1301487/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:65805/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1426798/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1408173/60‑1 (MQ=255)  aCGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:510261/60‑1 (MQ=255)   cGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1113163/59‑1 (MQ=255)          aTGGGCACGATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1612298/52‑1 (MQ=255)                  gATTTTGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:1184196/44‑1 (MQ=255)                    ttttGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:504302/42‑1 (MQ=255)                     tttGTCGCTGGTGCCGACGAACATCGTGGCGTCGATggcga  <  1:662146/41‑1 (MQ=255)                                             |                 CACGGCATTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGA  >  W3110S.gb/4298481‑4298541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑