Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 498,972 G→T L76I (CTC→ATC)  aes ← acetyl esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb498,9720GT100.0% 379.6 / NA 132L76I (CTC→ATC) aesacetyl esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (132/0);  total (132/0)

Only 100 of 132 total aligned reads displayed.

ATAAAATAGCGTCGCTGGGCTATCTGGCTGCGGACAAAAGAGACGTGTTTCCACCTGCCCAT  >  W3110S.gb/498931‑498992
                                         |                    
ataaaataGCGTCGCCGGCCTATCTGGCTGTGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1601686/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGtttt             >  1:89279/1‑50 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCCCCTGCCCat  >  1:657193/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCAcc         >  1:1337312/1‑55 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGccc    >  1:564876/1‑60 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:367530/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:591109/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:581685/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:557265/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:551194/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:489979/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:488002/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:42589/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:416434/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:412207/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:377084/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:610147/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:365380/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:34728/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:337487/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:335826/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:308805/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:289472/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:286717/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:267624/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:25821/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:245325/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:763921/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:986646/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:980676/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:955392/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:9438/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:928640/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:897850/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:871977/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:838941/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:838179/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:784619/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:770029/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:601991/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:748298/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:730257/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:690992/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:690361/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:655881/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:650785/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:63208/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:61809/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:614482/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1003808/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1220794/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1402894/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1390916/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1370544/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:136212/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1341904/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1334413/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1331811/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1302414/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1285478/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1231735/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1221183/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:201664/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1219496/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1210898/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1181650/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1175993/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:117365/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1133594/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1127854/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1106695/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1064876/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1012523/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1000555/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1457169/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:213117/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:197036/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:195021/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1639937/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1638047/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1635369/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1633441/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1618960/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1594573/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1590848/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1588161/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1545733/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:153475/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1504021/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1498854/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1472665/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1464743/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:1461813/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCat  >  1:14170/1‑62 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCa   >  1:1528717/1‑61 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCa   >  1:1301905/1‑61 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCa   >  1:1489590/1‑61 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCa   >  1:514753/1‑61 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTGTTTTCACCTGCCCa   >  1:989694/1‑61 (MQ=255)
ataaaataGCGTCGCCGGCCTATCTGGCTGCGGATAAAAGATACGTCTTTTCACCTGACCat  >  1:1310887/1‑62 (MQ=255)
                                         |                    
ATAAAATAGCGTCGCTGGGCTATCTGGCTGCGGACAAAAGAGACGTGTTTCCACCTGCCCAT  >  W3110S.gb/498931‑498992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑